阵列式柔性纸基SERS细菌检测芯片的制备

陈李; 李丹阳; 杨峰; 徐溢; 李顺波

doi:10.3788/OPE.20202801.0110

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阵列式柔性纸基SERS细菌检测芯片的制备

微纳技术与精密机械 | 更新时间：2020-08-13

- 阵列式柔性纸基SERS细菌检测芯片的制备
- Fabrication of array flexible paper-based SERS microarray for bacterial detection
- 光学精密工程 2020年28卷第1期页码：110-118
- 作者机构：
  
  1.重庆大学光电技术与系统教育部重点实验室，新型微纳器件与系统技术重点学科实验室，重庆 400044；
  2.中国科学院传感器技术国家重点实验室，上海 200050
- 作者简介：
  
  [ "陈李(1981-)，男，重庆人，博士，副教授，博士生导师，长期从事微纳传感器与精密仪器研究，研究兴趣包括MEMS传感器、MEMS加工工艺、SERS生化检测技术、荧光检测和分析技术等。Email：CL2009@cqu.edu.cn" ]
  [ "李丹阳(1996-)，女，河南商丘人，硕士研究生，主要从事SERS生化检测技术方面的研究。Email：13093770060@163.com" ]
- 基金信息：
  
  国家重点研发计划资助项目(2018YFB2002302);国家自然科学基金资助项目(61971074);中央高校基本科研业务费(2019CDYGYB003)
- DOI：10.3788/OPE.20202801.0110
  中图分类号： O657.37; R378.2
- 收稿日期：2019-06-27，
  
  录用日期：2019-8-25，
  
  纸质出版日期：2020-01-15
- 稿件说明：
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陈李, 李丹阳, 杨峰, 等. 阵列式柔性纸基SERS细菌检测芯片的制备[J]. 光学精密工程, 2020,28(1):110-118.

Li CHEN, Dan-yang LI, Feng YANG, et al. Fabrication of array flexible paper-based SERS microarray for bacterial detection[J]. Optics and precision engineering, 2020, 28(1): 110-118.
陈李, 李丹阳, 杨峰, 等. 阵列式柔性纸基SERS细菌检测芯片的制备[J]. 光学精密工程, 2020,28(1):110-118. DOI： 10.3788/OPE.20202801.0110.

Li CHEN, Dan-yang LI, Feng YANG, et al. Fabrication of array flexible paper-based SERS microarray for bacterial detection[J]. Optics and precision engineering, 2020, 28(1): 110-118. DOI： 10.3788/OPE.20202801.0110.

摘要

为实现细菌的快速、低成本、高通量检测，设计并制备了一种阵列式柔性纸基SERS检测芯片。首先制备银溶胶，再利用激光打印和碳粉的疏水性，在纸基上分别构造隔离区与阵列检测区。在检测区循环滴加银溶胶，由于碳粉的疏水特性，将银溶胶液滴限制在检测区范围。一定温度下，银溶胶自然干燥即形成阵列式活性SERS检测芯片。利用罗丹明6G作为探针分子对纸基SERS芯片进行了表征，测试结果表明该芯片的检测限为10

-8

mol/L

重复性测试的RSD约为11.85%。大肠杆菌SERS测试实验表明，该芯片可快速获得大肠杆菌的拉曼特征峰，无需对样本进行标记或复杂的前处理。该柔性纸基SERS芯片结构简单、制作快速、成本低廉，阵列结构可实现多参数的同时测量，有望应用于致病菌的直接、快速检测。

Abstract

To achieve rapid

low-cost

and high-throughput detection of bacteria

an array flexible paper-based Surface-Enhanced Raman Scattering(SERS) detection chip was designed and fabricated. First

silver sol was prepared

and then the isolation area and the array detection area were constructed on paper base using the hydrophobicity of laser printing and carbon powder. Given the hydrophobic property of carbon powder

silver sol droplets were confined to the detection area. At a certain temperature

the silver sol dries naturally to form an array of active SERS detection chip. The paper-based SERS chip was characterized by Rhodamine 6G as probe molecule. The test results show that the detection limit of the chip is 10

-8

mol/L

and the RSD of the repeatability test is about 11.85%. The SERS test of Escherichia coli(E. coli) shows that the chip can quickly obtain the Raman characteristic peaks of E. coli without labeling or complicated pretreatment. The advantages of the flexible paper-based SERS chip include simple structure

quick fabrication

and low cost. The array structure can realize simultaneous measurement of multiple parameters

and is expected to be used for direct and rapid detection of pathogenic bacteria.

关键词

Keywords

references

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